上傳人: 上海起發(fā)實驗試劑有限公司 |大?。?28.75KB|瀏覽:2254次|下載:2次|上傳:2018-09-29
文檔簡介 pPIC9K型號載體名稱出品公司載體用途VJI0299pPIC9KInvitrogen酵母表達載體產(chǎn)品參數(shù):測序引物序列:5'd[GCAAATGGCATTCTGACATCC]3'測序引物:3'AOX1載體抗性:氨芐青霉素(Ampicillin)載體描述:ThepPIC9KvectorcarriesthekanamycinresistancegenethatconfersresistancetoGeneticinReagentinPichia.SpontaneousgenerationofmultipleinsertioneventscanbeidentifiedbyresistancetoincreasedlevelsofGeneticinReagent.Pichiatransformantsareselectedonhistidine-deficientmediumandscreenedfortheirlevelofresistancetoGeneticinReagent.TheabilitytogrowinhighconcentrationsofGeneticinindicatesthatmultiplecopiesofthekanamycinresistancegeneandthegeneofinterestareintegratedintothegenome.ThepPIC9Kvectordirectssecretionofexpressedproteins.質(zhì)粒圖譜:上海起福生物科技有限公司聯(lián)系人:楊建輝400電話:4006551678辦公電話:021-50724187傳真:021-50724961*8006手機:15921799099企業(yè)QQ:4006551678網(wǎng)址:http://www.qfbio.com/地址:上海市浦東新區(qū)繡川路561號1102室
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文檔簡介 pPIC9K型號載體名稱出品公司載體用途VJI0299pPIC9KInvitrogen酵母表達載體產(chǎn)品參數(shù):測序引物序列:5'd[GCAAATGGCATTCTGACATCC]3'測序引物:3'AOX1載體抗性:氨芐青霉素(Ampicillin)載體描述:ThepPIC9KvectorcarriesthekanamycinresistancegenethatconfersresistancetoGeneticinReagentinPichia.SpontaneousgenerationofmultipleinsertioneventscanbeidentifiedbyresistancetoincreasedlevelsofGeneticinReagent.Pichiatransformantsareselectedonhistidine-deficientmediumandscreenedfortheirlevelofresistancetoGeneticinReagent.TheabilitytogrowinhighconcentrationsofGeneticinindicatesthatmultiplecopiesofthekanamycinresistancegeneandthegeneofinterestareintegratedintothegenome.ThepPIC9Kvectordirectssecretionofexpressedproteins.質(zhì)粒圖譜:上海起福生物科技有限公司聯(lián)系人:楊建輝400電話:4006551678辦公電話:021-50724187傳真:021-50724961*8006手機:15921799099企業(yè)QQ:4006551678網(wǎng)址:http://www.qfbio.com/地址:上海市浦東新區(qū)繡川路561號1102室
標簽: 相關產(chǎn)品