(Qiagen 205111) : ; (Qiagen 205111) , , ,elisa ,
(Qiagen 205111)

1. 10-20 20 2000-3000 /
2. EDTA 10-20 20 2000-3000 /
3. 20 2000-3000 /
4. 20 2000-3000 / PBS PH7.2-7.4 100 /ml 20 2000-3000 /
5. PBS PH7.4 2-8 PBS PH7.4 20 2000-3000 /
6. -20 .
7. NaN3 NaN3 HRP
(Qiagen 205111)

1. 10 100 l 50 l 100 l 50 l 50 l 50 l 50ul 50 l 50 l 50 l 50 l 50 l 50 l 6 pmol/L 4 pmol/L 2 pmol/L 1 pmol/L 0.5 pmol/L
2. 40 l 10 l 5
3. 37 30
4. 30 48T 20 30 48T 20
5. 30 5
6. 50 l
7. 3
8. 5
9. A50 l B50 l 37 15 .
10. 50 l
11. 450nm OD 15
(Qiagen 205111)

1 15-30
2
3 5
4 OD OD n n 5
5
6
7 .
8
9
10.
(Qiagen 205111)
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